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mcherry α tubulin  (Addgene inc)


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    Structured Review

    Addgene inc mcherry α tubulin
    Mcherry α Tubulin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmcherry+%CE%B1+tubulin+ires+puro2/pmCherry_a_tubulin_IRES_puro2+(Plasmid+%2321043)/bio_rxiv__2024__07__05__602013-272-10-21
    Average 91 stars, based on 13 article reviews
    mcherry α tubulin - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: GTPase of the Immune-Associated Nucleotide Protein 5 Regulates the Lysosomal Calcium Compartment in T Lymphocytes.
    Article Snippet: Secondary antibodies for fluorescence microscopy were purchased from Thermo Fisher Scientific (Waltham, MA, USA). .. Plasmids pmEGFP_α_tubulin_IRES_puro2b (Addgene plasmid # 21042) and pmCherry_α_tubulin_IRES_puro2 (Addgene 11 Serrano et al. GIMAP5 Regulates Lysosomal Calcium Frontiers in Immunology | www.frontiersin.org February 2017 | Volume 8 | Article 94 plasmid # 21043) were kind gifts of Dr. Daniel Gerlich (70). .. EGFP-IC2-FL (Addgene plasmid # 51409) was a gift from Dr. Trina Schroer (71).

    Article Title: GTPase of the Immune-Associated Nucleotide Protein 5 Regulates the Lysosomal Calcium Compartment in T Lymphocytes
    Article Snippet: Secondary antibodies for fluorescence microscopy were purchased from Thermo Fisher Scientific (Waltham, MA, USA). .. Plasmids pmEGFP_α_tubulin_IRES_puro2b (Addgene plasmid # 21042) and pmCherry_α_tubulin_IRES_puro2 (Addgene plasmid # 21043) were kind gifts of Dr. Daniel Gerlich ( ). .. EGFP-IC2-FL (Addgene plasmid # 51409) was a gift from Dr. Trina Schroer ( ).

    Stable Transfection:

    Article Title: Dynamic Kinetochore Size Regulation Promotes Microtubule Capture and Chromosome Biorientation in Mitosis
    Article Snippet: To generate stably integrated HeLa FlpIN, with LAP -tagged genes stably integrated in the FRT site and TetR inducible, pCDNA5-constructs were co-transfected with pOG44 recombinase in a 1:9 and kept in Hygromycin (Roche, 10843555001) selection for three weeks. .. To generate stably expressing mCherry-Tubulin and DARPIn GFP -mCherry-MPS1 Δ200 Hela FlpIn cell lines, pmCherry-α-tubulin-IRES-puro2 (Addgene #21043) or pCDNA4-DARPIn GFP -mCherry-Mps1 Δ200 variants were transfected and selected in Puromycin (Sigma, P7255) for three weeks. ..

    Expressing:

    Article Title: Dynamic Kinetochore Size Regulation Promotes Microtubule Capture and Chromosome Biorientation in Mitosis
    Article Snippet: To generate stably integrated HeLa FlpIN, with LAP -tagged genes stably integrated in the FRT site and TetR inducible, pCDNA5-constructs were co-transfected with pOG44 recombinase in a 1:9 and kept in Hygromycin (Roche, 10843555001) selection for three weeks. .. To generate stably expressing mCherry-Tubulin and DARPIn GFP -mCherry-MPS1 Δ200 Hela FlpIn cell lines, pmCherry-α-tubulin-IRES-puro2 (Addgene #21043) or pCDNA4-DARPIn GFP -mCherry-Mps1 Δ200 variants were transfected and selected in Puromycin (Sigma, P7255) for three weeks. ..

    Transfection:

    Article Title: Dynamic Kinetochore Size Regulation Promotes Microtubule Capture and Chromosome Biorientation in Mitosis
    Article Snippet: To generate stably integrated HeLa FlpIN, with LAP -tagged genes stably integrated in the FRT site and TetR inducible, pCDNA5-constructs were co-transfected with pOG44 recombinase in a 1:9 and kept in Hygromycin (Roche, 10843555001) selection for three weeks. .. To generate stably expressing mCherry-Tubulin and DARPIn GFP -mCherry-MPS1 Δ200 Hela FlpIn cell lines, pmCherry-α-tubulin-IRES-puro2 (Addgene #21043) or pCDNA4-DARPIn GFP -mCherry-Mps1 Δ200 variants were transfected and selected in Puromycin (Sigma, P7255) for three weeks. ..



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